SeCYS

DOUBLE SELENOCYSTEINE AND SELENOMETHIONINE LABELING OF PROTEINS

IN E. coli AUXOTROPHIC STRAIN

BL21(DE3) selB::kan cys51E 

The protocol achieves high level of biosynthetic incorporations and could be applied to any sulphur containing protein, even those containing disulphide bridges.

Other applications

in NMR:  isotopic labeling  (¹³C,²H,¹⁵N)cysteine,

in enzymology: probing active-site cysteine residues, ...

The biosynthetic incorporation of selenocysteine and/or 

selenomethionine into recombinant proteins have been exploited to produce  heavy-atom
derivatives for the elucidation of
their 3D structures  by

X-ray crystallography.

M.P. Strub,

F. Hoh,

J.F. Sanchez,

A. Aumelas,

A. Böck,

C. Dumas.