wTSA-CRAFT description

Thermal Shift Assay - analysis software:

This server provides a user-friendly interface to the TSA-CRAFT software developed by Po-Hsien Lee et al,. SLAS DISCOVERY 2019.
This implementation, as a dedicated web server, is under agreement with the authors.

Publication link: Lee P-H, Huang XX, Teh BT, Ng L-M. TSA-CRAFT: A Free Software for Automatic and Robust Thermal Shift Assay Data Analysis. SLAS DISCOVERY: Advancing the Science of Drug Discovery. 2019;24(5):606-612. doi: 10.1177/2472555218823547
Access to source code: https://sourceforge.net/projects/tsa-craft
TSA-Craft user manual: Here

Usage:

Webserver user manual: wTSA-Craft manual

I. TSA - Analysis form:
-> Screening Name : Enter your project name (optional)
-> Thermal Shift Data : CSV file containing wells assays data (mandatory)
-> Well Annotation: csv file containing annotation of wells (optional)
-> Graph overlay: Add up to 8 wells to be combined in a graph (optional)
-> Press "Launch analysis"!

II. Raw RTqPCR outputs data to TSA input files:
-> Dissociation file (mandatory)
-> Amplification file (mandatory)