The program T.I.T.O. (Tool for Incremental Threading Optimisation) evaluates the compatibility of an amino acid sequence (or a set of aligned sequences) with a known 3D structure. )his step is important for fold recognition and selection of the most compatible template(s). This programs helps one to validate or refine a structural alignment (Labesse & Mornon, 1998, Bioinformatics). A T.I.T.O. run usually takes only a few seconds (at most a few minutes) Citation Help Download T.I.T.O. also helps one to prepares input Files for MODELLER